AN EMBEDDING TECHNIQUE FOR ELECTRON MICROSCOPY USING EPON 812

An Embedding Technique for Electron Microscopy Using Epon 812

E p o x y resins h a v e been es tab l i shed as des i rab le e m b e d d i n g m e d i a for e lec t ron m i c r o s c o p y (1-5) . F inck (6) desc r ibed a n epoxy resin, E P O N 812 (Shell) t , w h i c h in a d d i t i o n to its o t he r des i rab le charac te r i s t ics , is also a p p r e c i a b l y misc ib le w i th wate r . H e no t ed t h a t p r o l o n g e d a lcohol d e h y d r a...

Araldite as an Embedding Medium for Electron Microscopy

Epoxy resins are suitable media for embedding for electron microscopy, as they set uniformly with virtually no shrinkage. A mixture of araldite epoxy resins has been developed which is soluble in ethanol, and which yields a block of the required hardness for thin sectioning. The critical modifications to the conventional mixtures are the choice of a plasticized resin in conjunction with an alip...

Problems in Methacrylate Embedding for Electron Microscopy

During an investigation of the effect of ischemia on striated muscle as seen with the electron microscope it became evident that all degrees of disorientation and destruction of tissues were taking place, and it seemed likely that much of the damage occurred during the embedding process. Such damage made it difficult to proceed with experiments in which comparison of normal tissue with any path...

A technique for identifying areas of interest human breast tissue before embedding for electron microscopy.

Correlative light and electron microscopy of the frog adrenal gland cells using adjacent epon-embedded sections.

Correlative light and electron microscopy on the same cells of the adrenal gland of the frog, Rana nigromaculata, fixed in glutaraldehyde followed by osmium tetroxide, was done using the adjacent Epon embedded sections. Electron microscope observation revealed three different types of granule-filled secretory cells; the noradrenaline-storing cells (NA cells) filled with intensely dense and vary...